Total Coliform Bacteria A particular group of bacteria that are used as indicators of possible sewage pollution. This group includes coliforms that inhabit the intestine of warm-blooded animals and those that inhabit soils. They are characterized as aerobic or facultative anaerobic, gram-negative, nonspore-forming, rod-shaped bacteria that ferment lactose with gas formation within 48 hours at 35 degrees centigrade. In the laboratory, these bacteria are defined as all the organisms that produce colonies with a golden-green metallic sheen within 24 hours when incubated at 35 degrees centigrade plus or minus 1 degree centigrade on M-Endo medium (nutrient medium for bacterial growth). Their concentrations are expressed as the number of colonies per 100 mL of sample.
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